sEPCR試劑盒說明書檢測范圍:
人、綿羊、小鼠、大鼠、豬、兔、山羊、牛、馬、豬、其它動物細胞因子、植物細胞因子、骨代謝、細胞凋亡、激素內(nèi)分泌、活性多肽、肝纖維化、自身抗體、血栓與止血、腫瘤、自身抗體科研Elisa檢測試劑盒。
服務:免費代測,免費索取產(chǎn)品說明書、免費技術(shù)指導等。
檢測種屬:人、大小鼠、兔、羊、猴、豬、豚鼠ELISA檢測試劑盒等種屬。
ELISA常用的方法:雙抗體夾心法、間接法、競爭法以及BAS-ELISA等。
存放環(huán)境:2-8℃,避光防潮保存。
產(chǎn)品名稱:sEPCR試劑盒說明書
英文縮寫:sEPCR
性狀:盒裝液體。
保存溫度: 2~8℃。
檢測范圍:見說明書靈敏度(以隨貨英文說明書為主)。
說明書:說明書隨貨發(fā)送,您也可以直接聯(lián)系我司在線銷售人員索取。
特點:靈敏性高,高效性,吸附均勻,吸附性好,回收利用率高,是一款可以讓您放心選購的elisa試劑盒產(chǎn)品。
用途:用于測定人血清、血漿、組織及相關(guān)液體樣本中的含量或活性。
sEPCR試劑盒說明書樣品收集、處理及保存:
1. 細胞培養(yǎng)上清:適用于檢測體外培養(yǎng)的細胞分泌性成份。用無菌管收集細胞上清液,以1000×g離心15分鐘,收集上清。
2. 細胞:用PBS反復洗滌細胞3次,調(diào)整細胞濃度達到104 -106 /ml 左右,通過反復凍融,使細胞破壞并放出細胞內(nèi)成份,或 者細胞超聲粉碎,離心取上清液檢測。
3. 血清:在室溫下,血液自然凝固,以1000×g離心15分鐘,取上清待測。
4. 血漿:應根據(jù)標本的要求選擇EDTA 或檸檬酸鈉作為抗凝劑,混合后靜置10-20 分鐘后,以1000×g離心15分鐘,收集上 清。
5. 體液:包括胸腹水、腦脊液,分泌物等。使用不含熱原和內(nèi)毒素的離心管收集,以1000×g離心15分鐘,收集上清。
6. 組織標本:切取組織標本,稱取重量0.5mg,加入500ul 的PBS,用手工或勻漿器,或超聲破碎儀將標本勻漿,以2000-3000 rmp離心20 分鐘,收集上清進行檢測。
7. 樣品中不能含有NaN3,因NaN3 抑制辣根過氧化物酶的(HRP)活性。
8. 保存:如果樣品不能立即檢測,應將其分裝,-70 ℃保存,避免反復冷凍。保存過程中如出現(xiàn)沉淀,應再次離心。血液標 本盡可能的不要使用溶血或高血脂血。如果血清中含大量顆粒,檢測前先離心或過濾。不要在37℃或更高的溫度加熱解 凍。應在室溫下解凍并確保樣品均勻地充分解凍。
sEPCR試劑盒說明書樣本實驗前準備:
elisa試劑盒液體樣本:包括血清、血漿、尿液、胸腹水、腦脊液、細胞 培養(yǎng)上清等。
(1)血清
室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。 仔細收集上清。保存過程中如有沉淀形成,應再次離心。
(2)血漿:
應根據(jù)標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分 鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如 有沉淀形成,應再次離心。
(3)尿液:
用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保 存過程中如有沉淀形成,應再次離心。胸腹水、腦脊液參照此實行。
(4)細胞培養(yǎng)上清:
檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分 )。仔細收集上清。
(5)培養(yǎng)細胞
檢測細胞內(nèi)的成份時,用PBS(PH7.2-7.4)稀 釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復凍融或加入組織蛋白萃 取試劑,以使細胞破壞并放出細胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
(6)組織標本
切割標本后,稱取重量。加入一定量的PBS, PH7.4。用液氮迅速冷凍保存?zhèn)溆谩吮救诨笕匀槐3?-8℃的溫度。加入 一定量的PBS(PH7.4),或組織蛋白萃取試劑, 用手工或勻漿器將標本勻漿 化。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。分裝后一份待檢 測,其余冷凍備用。
GOY-00019164Anti-E2F5 轉(zhuǎn)錄因子E2F-5抗體特價促銷規(guī)格: 0.2ml
GOY-00019165Anti-E2F6 轉(zhuǎn)錄因子E2F6抗體特價促銷規(guī)格: 0.1ml
GOY-00019166Anti-E2 tag E2 tag標簽抗體特價促銷規(guī)格: 0.1ml
GOY-00019167Anti-HPV16 E7 protein 人瘤病毒16型E7抗體特價促銷規(guī)格: 0.2ml
GOY-00019168Anti-EAAT1/Glast 膠質(zhì)細胞谷氨酸運載蛋白1抗體特價促銷規(guī)格: 0.1ml
GOY-00019169Anti-EAAT2 膠質(zhì)細胞谷氨酸運載蛋白2抗體特價促銷規(guī)格: 0.2ml
GOY-00019170Anti-EAAT3 膠質(zhì)細胞谷氨酸運載蛋白3抗體特價促銷規(guī)格: 0.1ml
GOY-00019171Anti-EBNA 3A EB病毒核抗原-3A抗體特價促銷規(guī)格: 0.2ml
GOY-00019172Anti-ECE1 內(nèi)皮素轉(zhuǎn)化酶1抗體特價促銷規(guī)格: 0.1ml
GOY-00019173Anti-ECE2 內(nèi)皮素轉(zhuǎn)化酶2抗體特價促銷規(guī)格: 0.2ml
GOY-00019174Anti-ECG2 食道癌相關(guān)基因抗體特價促銷規(guī)格: 0.1ml
GOY-00019175Anti-ECM1 細胞外基質(zhì)蛋白1抗體特價促銷規(guī)格: 0.2ml
GOY-00019176Anti-ECP 嗜酸性粒細胞陽離子蛋白抗體特價促銷規(guī)格: 0.2ml
GOY-00019177Anti-ED-1 外胚層發(fā)育不良抗體特價促銷規(guī)格: 0.1ml
人可溶性血管內(nèi)皮細胞蛋白C受體(sEPCR)ELISA試劑盒
本試劑盒僅供研究使用
試劑盒組成 | 96孔配置 | 48孔配置 | 備注 |
---|---|---|---|
微孔酶標板 | 8孔×12條 | 8孔×6條 | 無 |
標準品 | 0.3mL×6管 | 0.3mL×6管 | 無 |
樣本稀釋液 | 6mL | 3mL | 無 |
檢測抗體-HRP | 10mL | 5mL | 無 |
20×洗滌緩沖液 | 25mL | 15mL | 按說明書進行稀釋 |
底物A | 6mL | 3mL | 無 |
底物B | 6mL | 3mL | 無 |
終止液 | 6mL | 3mL | 無 |
封板膜 | 2張 | 2張 | 無 |
說明書 | 1份 | 1份 | 無 |
自封袋 | 1個 | 1個 | 無 |
HRH4/GPCR105抗體,組織胺H4受體抗體
【規(guī) 格】:100ul,200ul,1mg,5mg,10mg【濃 度】:1mg/ml【克隆類型】:兔來源為polyclonal,鼠來源為monoclonal【研究領(lǐng)域】:腫瘤,細胞生物,免疫學,染色質(zhì)和核信號,微生物學,細胞分化,表觀遺傳學【純 度】:98%【性 狀】:Lyophilized or Liquid【產(chǎn)品應用】: 應用領(lǐng)域(僅適用于科研實驗): Western blotting、Immunohistocry、ELISA、IF、IHC-P、IHC-F等,具體適用的實驗和針對的物種請來電或QQ咨詢。公司提供Elisa實驗配對抗體抗原,需要請電話或QQ咨詢!颈 存】:-20℃【保存條件】:Store at -20 °C for one year. The lyophilized powder is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Upon reconstituted, aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.【保 質(zhì) 期】:2年【相關(guān)標記抗體】:HRP標記抗體,Biotin標記抗體,Gold標記抗體,RBITC標記抗體,AP標記抗體,FITC標記抗體,Cy3標記抗體,Cy5標記抗體,Cy5.5標記抗體,Cy7標記抗體,PE標記抗體,PE-Cy3標記抗體,PE-Cy5標記抗體,PE-Cy5.5標記抗體,PE-Cy7標記抗體,APC標記抗體,Alexa Fluor 350標記抗體,Alexa Fluor 488標記抗體,Alexa Fluor 555標記抗體,Alexa Fluor 647標記抗體 公司提供生物試劑,Elisa實驗配對抗體抗原,需要請電話或QQ咨詢?头娫挘15800667461,,客服QQ:1187669894,553124757。,elisa實驗抗體,抗原,蛋白及多肽,并提供相應的二抗產(chǎn)品。歡迎新老客戶咨詢訂購。
HRH4/GPCR105抗體,組織胺H4受體抗體
按理化性質(zhì)和生物學功能,可將其分為IgM、IgG、IgA、IgE、IgD五類。IgM抗體是免疫應答中首先分泌的抗體。它們在與抗原結(jié)合后啟動補體的級聯(lián)反應。它們還把入侵者相互連接起來,聚成一堆便于巨噬細胞的吞噬;IgG抗體激活補體,中和多種毒素。IgG持續(xù)的時間長,是唯一能在母親妊娠期穿過胎盤保護胎兒的抗體。他們還從乳腺分泌進入初乳,使新生兒得到保護;IgA抗體進入身體的黏膜表面,包括呼吸、消化、生殖等管道的黏膜,中和感染因子。還可以通過母乳的初乳把這種抗體輸送到新生兒的消化道黏膜中,是在母乳中含量最多,最為重要的一類抗體;IgE抗體的尾部與嗜堿細胞、肥大細胞的細胞膜結(jié)合。當抗體與抗原結(jié)合后,嗜堿細胞與肥大細胞釋放組織胺一類物質(zhì)促進炎癥的發(fā)展。這也是引發(fā)速發(fā)型過敏反應的抗體;IgD抗體的作用還不太清楚。它們主要出現(xiàn)在成熟的B淋巴細胞表面上,可能與B細胞的分化有關(guān)。(IgD于1995年從人骨髓瘤蛋白中發(fā)現(xiàn),分子量為175kD,主要由扁桃體、脾等處漿細胞產(chǎn)生,人血清中IgD濃度為3~40μg/ml,不到血清總Ig的1%,在個體發(fā)育中合成較晚。IgD鉸鏈區(qū)很長,且對蛋白酶水解敏感,因此IgD半衰期很短,僅2.8天。血清中IgD確切的免疫功能尚不清楚。在B細胞分化到成熟B細胞階段,除了表達SmIgD,抗原刺激后表現(xiàn)為免疫耐受。成熟B細胞活化后或者活化后或者變成記憶B細胞時,SmIgD逐漸消失。
HRH4/GPCR105抗體,組織胺H4受體抗體
本公司備有上萬種產(chǎn)品!所有生化試劑產(chǎn)品都具有類似以下產(chǎn)品價格絕對優(yōu)勢,質(zhì)量保證!歡迎新老客戶垂詢!本公司經(jīng)銷并研發(fā)代理近萬種抗體產(chǎn)品,保證蛋白抗原產(chǎn)品質(zhì)量,質(zhì)量穩(wěn)定,實驗效果明顯,代理眾多知名品牌抗體abcam,CST,R&D,santa等,貨期快,價格優(yōu)惠,歡迎垂詢訂購!上海微蒙生物長期供應免疫組化抗體、WB抗體、免疫組化試劑盒和抗體試驗所需全部相關(guān)試劑、熒光標記抗體、elisa抗體、單克隆抗體、多克隆抗體、各種標記的二抗IgG/IgM/IgD/IgA等科研實驗抗體。
抗體是具有4條多肽鏈的對稱結(jié)構(gòu),其中2條較長、相對分子量較大的相同的重鏈(H鏈);2條較短、相對分子量較小的相同的輕鏈(L鏈)。鏈間由二硫鍵和非共價鍵聯(lián)結(jié)形成一個由4條多肽鏈構(gòu)成的單體分子。輕鏈有κ和λ兩種,重鏈有μ、δ、γ、ε和α五種。 整個抗體分子可分為恒定區(qū)和可變區(qū)兩部分。在給定的物種中,不同抗體分子的恒定區(qū)都具有相同的或幾乎相同的氨基酸序列?勺儏^(qū)位于"Y"的兩臂末端。在可變區(qū)內(nèi)有一小部分氨基酸殘基變化特別強烈,這些氨基酸的殘基組成和排列順序更易發(fā)生變異區(qū)域稱高變區(qū)。高變區(qū)位于分子表面,最多由17個氨基酸殘基構(gòu)成,少則只有2 ~ 3個。高變區(qū)氨基酸序列決定了該抗體結(jié)合抗原抗原的特異性。一個抗體分子上的兩個抗原結(jié)合部位是相同的,位于兩臂末端稱抗原結(jié)合片段(antigen-binding fragment, Fab)。"Y"的柄部稱結(jié)晶片段(crystalline fragment,FC),糖結(jié)合在FC 上。
HRH4/GPCR105抗體,組織胺H4受體抗體
Anti-rabbit IgG, HRP-linked Antibody 1ml |
Akt Antibody 100ul |
NF-κB p65 (D14E12) XP Rabbit mAb 100ul |
beta-Actin (13E5) Rabbit mAb 100ul |
SignalStain Boost IHC Detection Reagent (HRP, Mouse) 15ml |
Phospho-Akt (Ser473) (D9E) XP Rabbit mAb 100uL |
E-Cadherin (24E10) Rabbit mAb 100ul |
Phospho-Akt (Ser473) (D9E) XP Rabbit mAb 40uL |
Phospho-p44/42 MAPK (T202/Y204) (D13.14.4E) XP RmAb 200uL |
p38 MAPK Antibody 200ul |
Anti-mouse IgG, HRP-linked Antibody 1ml |
RIPA Buffer (10X) 15ml |
Cell Lysis Buffer (10X) 15ml |
GAPDH (14C10) Rabbit mAb 100ul |
Phospho-p44/42 MAPK (T202/Y204) (D13.14.4E) XP RmAb 40uL |
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb 200ul |
Cleaved Caspase-3 (Asp175) Antibody 100ul |
EGF Receptor (D38B1) XP Rabbit mAb 100ul |
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb 100ul |
SAPK/JNK (56G8) Rabbit mAb 200ul |
Anti-Rabbit IgG(H+L),F(ab')2 Fragment AlexaFluor488Co 250ul |
Bcl-2 (50E3) Rabbit mAb 40ul |
Phospho-NF-kappa-B p65 (Ser536) (93H1) Rabbit mAb 100ul |
HRH4/GPCR105抗體,組織胺H4受體抗體 |
LY294002 (PI3 Kinase Inhibitor) 1.5mg |
Phospho-Met (Tyr1234/1235) (D26) XP Rabbit mAb 100ul |
Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb 40ul |
GAPDH (D16H11) XP(tm) Rabbit mAb 100ul |
Phospho-I-kappa-B-alpha (Ser32) (14D4) Rabbit mAb 100ul |
Akt (pan) (C67E7) Rabbit mAb 100uL |
SAPK/JNK (56G8) Rabbit mAb 40ul |
Caspase-3 (8G10) Rabbit mAb 100ul |
NF-kappa-B Pathway Sampler Kit 1Kit |
SignalStain(R) Boost IHC Detection Reagent 15ml |
p53 (7F5) Rabbit mAb 100ul |
AMPK-alpha Antibody 100ul |
Phospho-AMPK-alpha (Thr172) (40H9) Rabbit mAb 100ul |
Bax Antibody 100ul |
p44/42 MAPK (Erk1/2) Antibody 200ul |
GSK-3-beta (27C10) Rabbit mAb 100ul |
U0126 (MEK1/2 Inhibitor) 5mg |
mTOR (7C10) Rabbit mAb 100uL |
Phospho-p38 MAPK (Thr180/Tyr182) (D3F9)XP Rabbit mAb 40ul |
Akt (pan) (C67E7) Rabbit mAb 40ul |
Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb 100ul |
Phospho-p38 MAPK (Thr180/Tyr182) Antibody 200ul |
Phospho-SAPK/JNK (Thr183/Tyr185) Antibody 200ul |
PD98059 (MEK1 Inhibitor) 1.5mg |
Myc-Tag (71D10) Rabbit mAb 100ul |
DYKDDDDK Tag Antibody 100uL |
LC3B Antibody 100ul |
Bcl-2 Antibody 100ul |
FoxO1 (C29H4) Rabbit mAb 100ul |
Phospho-Akt (Thr308) (C31E5) Rabbit mAb 100uL |
Beclin-1 (D40C5) Rabbit mAb 40ul |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody 200ul |
Bcl-2 (50E3) Rabbit mAb 100ul |
HRH4/GPCR105抗體,組織胺H4受體抗體 |
LC3B (G40) Antibody 100ul |
Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody 100ul |
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb 40ul |
NF-kappa-B p65 (C22B4) Rabbit mAb 100ul |
SQSTM1/p62 Antibody 100ul |
Ezh2 (D2C9) XP Rabbit mAb 100ul |
Vimentin (D21H3) XP Rabbit mAb 100ul |
Blue Loading Buffer Pack 1Pack |
p53 Antibody 100ul |
beta-Catenin (6B3) Rabbit mAb 100ul |
Apoptosis Marker: Cleaved Caspase-3(Asp175) Western Kit 1Kit |
MAPK Family Antibody Sampler Kit 1Kit |
Topoisomerase IIα (D10G9) XP(R) Rabbit mAb |
AEBP2 (D7C6X) Rabbit mAb 100ul |
alpha-Tubulin (11H10) Rabbit mAb 100ul |
His-Tag (27E8) Mouse mAb 100ul |
PCNA (PC10) Mouse mAb 100ul |
Phospho-mTOR (Ser2448) Antibody 300uL |
ALK (D5F3) XP Rabbit mAb 100ul |
MMP2 Antibody 100uL |
Phospho-Akt (Ser473) (587F11) Mouse mAb 100uL |
Cytochrome C Antibody 100uL |
IkappaB-a (44D4) Rabbit mAb 100uL |
Cox2 Antibody 100uL |
Stat3 (79D7) Rabbit mAb 100uL |
Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb 100uL |
Phospho-mTOR (Ser2448) (D9C2) XP Rabbit mAb 100ul |
Red Loading Buffer Pack 1Pack |
NF-κB p65 (D14E12) XP Rabbit mAb 40ul |
LIN28A (D1A1A) XP(R) Rabbit mAb 100ul |
Phospho-p44/42 MAPK (Thr202/Tyr204) (E10) Mouse mAb 200uL |
Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb 40ul |
CREB (48H2) Rabbit mAb 100ul |
Phospho-p70 S6 Kinase (Thr389) Antibody 100ul |
Phospho-GSK-3-beta (Ser9) (5B3) Rabbit mAb 100ul |
Acetylated-Lysine Antibody 100ul |
beta-Catenin Antibody 100ul |
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 100ul |
ChIP-Grade Protein G Magnetic Beads 1ml |
HER3/ErbB3 (D22C5) XP(R) Rabbit mAb 100ul |
FoxO3a (75D8) Rabbit mAb 100ul |
p70 S6 Kinase (49D7) Rabbit mAb 100ul |
p21 Waf1/Cip1 (12D1) Rabbit mAb 100uL |
Phospho-mTOR (Ser2448) Antibody 100ul |
Cyclin D1 (92G2) Rabbit mAb 40ul |
Insulin Receptor b (4B8) Rabbit mAb 100uL |
Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb 100uL |
Phospho-CaMKII (Thr286) Antibody 100uL |
TGF-b Antibody 100uL |
Snail (C15D3) Rabbit mAb 40ul |
Phospho-Akt (Thr308) (244F9) Rabbit mAb 100uL |
PI3 Kinase p85 Antibody 100ul |
Phospho-p44/42 MAPK (T202/Y204) (197G2) Rabbit mAb 200uL |
p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb 100uL |
YAP Antibody 100uL |
c-Myc (D84C12) Rabbit mAb 100ul |
p38 MAPK (D13E1) XP Rabbit mAb 40ul |
Phospho-Stat3 (Tyr705) Antibody 100ul |
c-Jun (60A8) Rabbit mAb 100ul |
Phospho-I-kappa-B-alpha (Ser32/36) (5A5) Mouse mAb 100ul |
Caspase-8 (1C12) Mouse mAb 200ul |
Paclitaxel 1mg |
Phospho-MAPK Family Antibody Sampler Kit 1Kit |
Trichostatin A (TSA) 1mg |
Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile) 10 ml |
DAPI 1mg |
Anti-mouse IgG(H+L),F(ab)2 Fragment (Alexa Fluor) 250ul |
20X LumiGLO(R) Reagent and 20X Peroxide 25milliliterseach |
Effector Caspases and Substrates Antibody Sampler Kit1KIT |
NeuN(D3S3I) Rabbit mAb 100ul |
xCT blocking peptide 100ug 100ug |
MRPL11 (D68F2) XP Rabbit mAb 100ul |
Src (36D10) Rabbit mAb 100ul |
Phospho-HER2/ErbB2 (Tyr1221/1222) (6B12) Rabbit mAb 100ul |
MMP-9 (G657) Antibody 100ul |
Phospho-IRS-1 (Ser307) Antibody 100ul |
Phospho-VEGF Receptor 2 (Tyr1175) (19A10) Rabbit mAb 100ul |
Phospho-AMPK-alpha (Thr172) Antibody 100ul |
Phospho-AMPK-alpha (Thr172) (40H9) Rabbit mAb 40ul |
AMPK-alpha (23A3) Rabbit mAb 100ul |
GST (26H1) Mouse mAb 100ul |
Bim (C34C5) Rabbit mAb 100uL |
p21 Waf1/Cip1 (12D1) Rabbit mAb 40ul |
iNOS Antibody 100uL |
Insulin (C27C9) Rabbit mAb 100uL |
FAK Antibody 100uL |
eIF4H (D85F2) XP Rabbit mAb 100ul |
GFAP (GA5) Mouse mAb 100ul |
GRB10 Antibody 100uL |
HIF1-a Antibody 100uL |
Phospho-EGF Receptor (Tyr1068) (D7A5) XP Rabbit mAb 100ul |
MMP-9 Antibody 100uL |
PKM2 (D78A4) XP Rabbit mAb 100ul |
Phospho-Akt (Ser473) (193H12) Rabbit mAb 100ul |
Phospho-Akt (Ser473) (D9E) XP Rabbit mAb 300uL |
PI3 Kinase Class III (D9A5) XP Rabbit mAb 100ul |
HER2/ErbB2 (D8F12) XP Rabbit mAb 100ul |
Phospho-p44/42 MAPK (Thr202/Tyr204) (20G11) RmAb (IHC) 200uL |
Akt (pan) (11E7) Rabbit mAb 100ul |
Smad2 (D43B4) XP Rabbit mAb 100ul |
K63-linkage Specific Polyubiquitin (D7A11) Rabbit mAb 100ul |
Anti-rabbit IgG, HRP-linked Antibody 100 ul |
Met (D1C2) XP Rabbit mAb 100ul |
PhosphoPlus p44/42MAPK(Erk1/2)(Thr202/Tyr204)Ab Duet 1Kit |
p44/42 MAPK (Erk1/2) (3A7) Mouse mAb 100ul |
Phospho-p53 (Ser15) Antibody 100ul |
Phospho-GSK-3-beta (Ser9) Antibody 100ul |
PARP Antibody 100ul |
PTEN (138G6) Rabbit mAb 100ul |
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 40ul |
Calyculin A (Serine/Threonine Phosphatase Inhibitor) 10ug |
Rapamycin (FRAP/mTOR Inhibitor) 10nmol |
Apoptosis Sampler Kit 1Kit |
PathScan(R) Phospho-EGF Receptor (Tyr1068) Chemiluminescent |
BRM (D9E8B) XP Rabbit mAb 100ul |
SDHA (D6J9M) XP Rabbit mAb 100ul |
Cox2 (D5H5) XP Rabbit mAb 100ul |
SMARCAD1 Antibody mAb 100ul |
SirT6 (D8D12) Rabbit mAb 100ul |
USP Antibody Sampler Kit 1Kit |
EGF Receptor(E746-A750del Specific)(6B6) XP Rabbit mAb 100ul |
Phospho-Src Family (Tyr416) Antibody 100ul |
Rictor (53A2) Rabbit mAb 100ul |
Caspase-12 Antibody 100ul |
Toll-like Receptor 4 Antibody (Rodent Specific) 100ul |
HA-Tag (6E2) Mouse mAb 100ul |
VEGF Receptor 2 (55B11) Rabbit mAb 100ul |
p53 (1C12) Mouse mAb 100ul |
Pan-Calcineurin A Antibody 100ul |
NEDD8 (19E3) Rabbit mAb 100ul |
Bcl-xL Antibody 100ul |
Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb 100ul |
CHOP (L63F7) Mouse mAb 40ul |
Cyclin D1 Antibody 100uL |
Cyclin D1 (DCS6) Mouse mAb 40ul |
GFP (4B10) Mouse mAb 100uL |
Lck (D88) XP Rabbit mAb 100ul |
NF-kB p105/p50 Antibody 100uL |
Phospho-Smad2 (Ser465/467) Antibody 100uL |
Phospho-Smad2 (Ser245/250/255) Antibody 100uL |
Met (L41G3) Mouse mAb 100uL |
Phospho-PERK (Thr980) (16F8) Rabbit mAb 100uL |
EGF Receptor (L858R Mutant Specific) (43B2) Rabbit mAb 100ul |
PKM2 Antibody 100uL |
Jak1 (6G4) Rabbit mAb 100uL |
TBK1/NAK (D1B4) Rabbit mAb 100ul |
Cleaved Notch1 (Val1744) (D3B8) XP Rabbit mAb 100ul |
PI3 Kinase p110alpha (C73F8) Rabbit mAb 100uL |
PI3 Kinase p85 (19H8) Rabbit mAb 100uL |
EGF Receptor (D38B1) XP Rabbit mAb 40ul |
Histone H3 (D1H2) XP Rabbit mAb 100ul |
Met Antibody 100uL |
Phospho-p38 MAPK (Thr180/Tyr182) (12F8) RmAb (IHC) 200uL |
Lamin A/C (4C11) Mouse mAb 100ul |
I-kappa-B-alpha (L35A5) Mouse mAb 100ul |
CREB (D76D11) XP Rabbit mAb 100ul |
HSP70 Antibody 100uL |
beta-Actin Antibody 300ul |
beta-Actin (13E5) Rabbit mAb 300uL |
Bax (D2E11) Rabbit mAb 40ul |
c-Myc (D84C12) Rabbit mAb 40ul |
BACE (D10E5) Rabbit mAb 100ul |
Cleaved PARP (Asp214) (D64E10) XP Rabbit mAb 100ul |
SB203580 (p38 inhibitor) 5mg |
GSK-3-α/β (D75D3) XP Rabbit mAb 100ul |
Vimentin (D21H3) XP Rabbit mAb 40ul |
SignalSilence Control siRNA (Unconjugated) 150uL |
NF-κB p65 (L8F6) Mouse mAb 100ul |
Biotinylated Protein Ladder Detection Pack 325mini-blots |
Biotinylated Protein Ladder Detection Pack 65mini-blots |
EGF Receptor (D38B1) XP(R) Rabbit mAb (HRP Conjugate) 100ul |
DRP1 (D6C7) Rabbit mAb 100ul |
Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) 100ul |
ProLong Gold Antifade Reagent with DAPI 10ml |
Ki-67 (D2H10) Rabbit mAb (IHC Specific) 100ul |
StemLite iPS Cell Reprogramming Antibody Kit 1Kit |
Blimp-1/PRDI-BF1 (C14A4) Rabbit mAb 100ul |
MEK1/2 Antibody 100ul |
Phospho-Stat3 (Ser727) Antibody 40ul |
Stat3 (124H6) Mouse mAb 100ul |
Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb 100ul |
Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb 100ul |
SP1 (D4C3) Rabbit mAb 100ul |
c-Raf Antibody 100ul |
Acetylated-Lysine Antibody 300ul |
Phospho-FoxO1 (Ser256) Antibody 100ul |
Caspase-9 (C9) Mouse mAb 100ul |
Smad4 Antibody 100ul |
Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb 100ul |
Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb 100ul |
Cleaved PARP (Asp214) Antibody (Human Specific) 100ul |
PARP Antibody 40ul |
Slug (C19G7) Rabbit mAb 100ul |
Phospho(Ser) 14-3-3 Binding Motif Antibody 100ul |
Phospho(Ser/Thr) PKA Substrate Antibody 100ul |
Acetylated-Lysine Mouse mAb (Ac-K-103) 400ul |
GSK-3β (3D10) Mouse mAb 100ul |
Pro-Survival Bcl-2 Family Antibody Sampler Kit (Human Specif |
PathScan Immune Cell Signaling Antibody Array Kit 1Kit |
alpha-Synuclein (D37A6) XP(R) Rabbit mAb (Alexa Fluor(R) 555 |
DYKDDDDK Tag Antibody (Alexa Fluor 647 Conjugate) 100ul |
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP(R) R |
DRAQ5 50ul |
P-p44/42MAPK (Thr202/Tyr204)(D13.14.4E) XP RmAb(AF647) 500ul |
Anti-mouseIgG(H+L),F(ab')2Fragment(AlexaFluor488) 250ul |
Anti-rat IgG (H+L), (Alexa Fluor 488 Conjugate) 250ul |
alpha-Synuclein (D37A6) XP(R) Rabbit mAb (Alexa Fluor(R) 647 |
Sox2 (D6D9) XP(R) Rabbit mAb (Alexa Fluor(R) 647 Conjugate) |
Mouse Interleukin-10 (mIL-10) 10ug |
Mouse Vascular Endothelial Growth Factor-120 (mVEGF120 ) 10u |
alpha-Synuclein (D37A6) XP(R) Rabbit mAb (Alexa Fluor(R) 488 |
Phosphatase Inhibitor Cocktail (100X) 1ml |
PDGF Receptor alpha (D13C6) XP(R) Rabbit mAb (Alexa Fluor(R) |
EGF Receptor (D38B1) XP(R) Rabbit mAb (Magnetic Bead Conjuga |
Human Erythropoietin (hEPO) 50ug |
PathScan(R) Phospho-Insulin Receptor beta (panTyr) Sandwich |
PathScan Phospho-HER2/ErbB2 (Tyr1221/1222) ELISAKit 1Kit |
SignalStain Antibody Diluent 100ml |
SignalStain Antibody Diluent 25ml |
SignalStain Boost IHC Detection Reagent (HRP, Rabbit) 1ml |
SignalStain(R) Boost IHC Detection Reagent (HRP, Mouse) 1 ml |
Human Transforming Growth Factor β3 (hTGF-β3) 10ug |
Phospho-GSK-3-beta (Ser9) (D85E12) XP(R) Rabbit mAb (PE Conj |
Met (D1C2) XP(R) Rabbit mAb (Alexa Fluor(R) 488 Conjugate) 1 |
Phospho-Histone H3 (Ser10) (D2C8) XP(R) Rabbit mAb (Pacific |
Nanog (D73G4) XP(R) Rabbit mAb (Alexa Fluor(R) 594 Conjugate |
Keratin 17 (D73C7) Rabbit mAb (Alexa Fluor(R) 647 Conj |
Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Flu |
Human Interleukin-6 (hIL-6) 10ug |
Human Vascular Endothelial Growth Factor-121 50ug |
Human Leukemia Inhibitory Factor (hLIF) 50ug |
Human Stem Cell Factor (hSCF) 50ug |
Human Stem Cell Factor (hSCF)10ug |
Androgen Receptor (D6F11) XP(R) Rabbit mAb (Alexa Fluor(R) 5 |
Phospho-Rb (Ser807/811) (D20B12) XP(R) Rabbit mAb (Alexa Flu |
SimpleChIP(tm) Enzymatic Chromatin IP Kit (Agarose Beads) 1K |
Phospho-Histone H2A.X (Ser139) (20E3) RmAb (Alexa 488) 100uL |
Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP(R) R |
Mouse Insulin-like Growth Factor I (mIGF-I) 50ug |
P-Acetyl-CoA Carboxylase (Ser79) (D7D11) Rabbit mAb 100ul |
Phospho-Smad1/5 (Ser463/465) (D5B10) Rabbit(ChIP)mAb 100ul |
RNF20 (D6E10) XP(R) Rabbit mAb 100ul |
Glucocorticoid Receptor (D6H2L) XP Rabbit mAb 40ul |
Bak (D4E4) Rabbit mAb 100ul |
Caveolin-1 (D46G3) XP(R) Rabbit mAb (HRP Conjugate) 100ul |
His-Tag (D3I1O) XP? Rabbit mAb 100ul |
Phospho-CaMKII(Thr286)(D21E4) Rabbit mAb 100ul |
Smad2/3 Antibody Sampler Kit 1Kit |
CDK4 (D9G3E) Rabbit mAb 100ul |
FoxO3a (D19A7) Rabbit mAb 100ul |
Phospho-ATM (Ser1981) (D25E5) Rabbit mAb 100ul |
iNOS (D6B6S) Rabbit mAb 100ul |
NLRP3 (D2P5E) Rabbit mAb 100ul |
Lamin B1 (D9V6H) Rabbit mAb 100ul |
eIF4E (C46H6) Rabbit mAb 100ul |
Phospho-IKKalpha (Ser176)/IKKbeta (Ser177) Rabbit mAb 100uL |
Src Antibody 100ul |
RhoA (67B9) Rabbit mAb 40ul |
GAPDH (14C10) Rabbit mAb 300ul |
Src (32G6) Rabbit mAb 100ul |
alpha-N-Catenin Antibody 100ul |
ATGL Antibody 100ul |
Axin2 (76G6) Rabbit mAb 100ul |
Myc-Tag Antibody 100ul |
Myc-Tag (9B11) Mouse mAb 100ul |
Raptor (24C12) Rabbit mAb 40ul |
C/EBP-alpha Antibody 100ul |
Phospho-Chk1 (Ser345) (133D3) Rabbit mAb 40ul |
Phospho-c-Jun (Ser63) (54B3) Rabbit mAb 100ul |
His-Tag Antibody 100ul |
Cdc42 (11A11) Rabbit mAb 100ul |
SirT1 (C14H4) Rabbit mAb 100ul |
TrkA (14G6) Rabbit mAb 100ul |
CDK2 (78B2) Rabbit mAb 100ul |
Phospho-CDK2 (Thr160) Antibody 100ul |
DLL4 Antibody 100ul |
Acetyl-Histone H4 (Lys12) Antibody 100ul |
HP1-gamma Antibody 100ul |
GST (91G1) Rabbit mAb 100ul |
Atg7 Antibody 100ul |
Histone H3 Antibody (ChIP Formulated) 100ul |
Stat4 (C46B10) Rabbit mAb 100ul |
Phospho-Chk2 (Ser19) Antibody 100ul |
p35/25 (C64B10) Rabbit mAb 100ul |
p70 S6 Kinase (49D7) Rabbit mAb 40ul |
FEN-1 Antibody 100ul |
Bcl-xL (54H6) Rabbit mAb 40ul |
Bcl-xL (54H6) Rabbit mAb 100ul |
Bim Antibody 100ul |
Phospho-PLC-gamma-1 (Tyr783) Antibody 100ul |
Oct-4 (C30A3) Rabbit mAb 100ul |
Phospho-4E-BP1(Thr37/46)(236B4) Rabbit mAb 40ul |
Bcl-2 Antibody (Human Specific) 100ul |
ATM (D2E2) Rabbit mAb 100ul |
CTCF Antibody 100ul |
EpCAM (VU1D9) Mouse mAb 100uL |
Akt1 (C73H10) Rabbit mAb 100uL |
mTOR Antibody 100ul |
Phospho-mTOR (Ser2448) (49F9) Rabbit mAb (IHC Specific) 100u |
Acetyl-NF-kB p65 (Lys310) Antibody 100uL |
LKB1 (D60C5) Rabbit mAb 100uL |
PDK1 Antibody 100uL |
Phospho-Met (Tyr1234/1235) (D26) XP Rabbit mAb 40ul |
IRS-2 (L1326) Antibody 100uL |
Smad2/3 Antibody 100uL |
Phospho-Met (Tyr1234/1235) Antibody 100uL |
Met (25H2) Mouse mAb 300uL |
CDK6 (DCS83) Mouse mAb 100uL |
Ezrin Antibody 100uL |
Moesin Antibody 100uL |
Moesin (Q480) Antibody 100uL |
PDGF Receptor a Antibody 100uL |
PERK (C33E10) Rabbit mAb 100ul |
Caveolin-1 Antibody 100uL |
Phospho-c-Jun (Ser73) (D47G9) XP Rabbit mAb 40ul |
Phospho-FAK (Tyr397) Antibody 100uL |
ROS1 (D4D6) Rabbit mAb 40ul |
ROS1 (D4D6) Rabbit mAb 100ul |
G9a/EHMT2 (C6H3) Rabbit mAb 100uL |
Phospho-Cofilin (Ser3) (77G2) Rabbit mAb 100uL |
ApoA5 (2G1H11) Mouse mAb 100ul |
Phospho-ALK (Tyr1604) Antibody 100uL |
CaMKII Antibody 100uL |
Phospho-Histone H3 (Ser10) (D2C8) XP Rabbit mAb 100ul |
TCF8/ZEB1 (D80D3) XP Rabbit mAb 100ul |
Myosin Va Antibody 100ul |
Hydroxy-HIF-1α (Pro564) (D43B5) XP Rabbit mAb 40ul |
Hydroxy-HIF-1α (Pro564) (D43B5) XP Rabbit mAb 100ul |
Notch3 (8G5) Rat mAb 100ul |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP( |
Connexin 43 Antibody 100uL |
DNMT3A (D23G1) Rabbit mAb 100ul |
Notch1 (D1E11) XP Rabbit mAb 100ul |
Acetyl CoA Carboxylase Antibody 100uL |
Myosin Light Chain 2 Antibody 100uL |
Phospho-Myosin Light Chain 2 (Thr18/Ser19) Antibody 40ul |
Phospho-Myosin Light Chain 2 (Ser19) Mouse mAb 100uL |
Acetyl-CoA Carboxylase (C83B10) Rabbit mAb 40ul |
p27 Kip1 (D69C12) XP Rabbit mAb 40ul |
TNF-a Antibody 100uL |
TGF-b (56E4) Rabbit mAb 100uL |
Beclin-1 Antibody 100uL |
E2F-1 Antibody 100uL |
Rig-I (D14G6) XP Rabbit mAb 100ul |
GFAT1 Antibody 100ul |
Phospho-FRS2-a (Tyr196) Antibody 100uL |
Snail (C15D3) Rabbit mAb 100uL |
LRP5 (D23F7) Rabbit mAb 100ul |
Phospho-Glycogen Synthase (Ser641) Antibody 100uL |
SOCS1 (A156) Antibody 100ul |
ROCK1 (C8F7) Rabbit mAb 100uL |
Phospho-Drosophila Akt (Ser505) Antibody 100uL |
Rabbit (DA1E) mAb IgG XP Isotype Control (Biotinylated) |
HSL Antibody 100uL |
EGR1 (15F7) Rabbit mAb 100ul |
MKK7 Antibody 100uL |
TPA (12-O-Tetradecanoylphorbol-13-Acetate) 1mg |
Phospho-β-Catenin (Ser675) (D2F1) XP Rabbit mAb 100ul |
EGF Receptor (D38B1) XP(R) Rabbit mAb 300ul |
Phospho-Stat5 (Tyr694) (D47E7) XP Rabbit mAb 100ul |
Synaptophysin Antibody 100ul |
ACE2 Antibody 100uL |
HSF1 Antibody 100uL |
UGT Antibody 100uL |
MTSS1 (P549) Antibody 100uL |
A-Raf Antibody 40ul |
CaMKII (pan) (D11A10) XP Rabbit mAb 100ul |
ARK5 Antibody 100uL |
Keratin 18 (DC10) Mouse mAb 100uL |
Mcl-1 Antibody 100uL |
Phospho-SAPK/JNK (T183/Y185) (98F2) Rabbit mAb 200uL |
Aurora A/AIK (1G4) Rabbit mAb 100uL |
TRAF2 Antibody (Human Specific) 100uL |
HER3/ErbB3 (1B2) Rabbit mAb 100uL |
Acetyl-CBP (Lys1535)/p300 (Lys1499) Antibody 100uL |
ULK1 (A705) Antibody 100ul |
Phospho-PKA C (Thr197) Antibody 100uL |
Caspase-8 (D35G2) Rabbit mAb 100ul |
COX IV Antibody 100uL |
Phospho-S6R Protein (Ser235/236) (D57.2.2E) XP RmAb 40uL |
VDAC Antibody 100uL |
Phospho-S6R Protein (Ser235/236) (D57.2.2E) XP RmAb 40uL
VDAC Antibody 100uL
產(chǎn)品中文:人可溶性血管內(nèi)皮細胞蛋白C受體(sEPCR)ELISA試劑盒
產(chǎn)品英文:Human soluble endothelial protein C receptor,sEPCR ELISA Kit
規(guī)格:96T 48T
價格:詢價
本公司elisa試劑盒可以提供免費代測服務,客戶自己提供代測樣本,七個工作日即可提供客戶所需原始數(shù)據(jù)。
如需要說明書請聯(lián)系我司在線客服
本公司長期代理各種種屬的elisa試劑盒(酶聯(lián)免疫法)同時還經(jīng)營各種一線品牌產(chǎn)品:sigma,amresco(大量現(xiàn)貨),Abcam,OXOID,Axygen試劑盒,Santa,invitrogen等知名品牌,貨期穩(wěn)定!
公司成立以來,一直秉著誠信、專業(yè)的經(jīng)營理念為每一位客戶提供真誠的服務。我們的服務理念是:做事不離誠信二字,恒古至今,唯有誠信才能贏得最后的勝利!感謝多年以來全國各個地方的兄弟姐妹們對本公司的大力支持!希望通過我們的不懈努力能為科研事業(yè)做出更大的貢獻!
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艾美捷科技有限公司,目前提供Abbkine高質(zhì)量抗體:二氫乳清酸脫氫酶 多克隆抗體,性價比非常高。Abbkine致力于為全球生命科學研究和醫(yī)療發(fā)展提供最新的產(chǎn)品和技術(shù)。Abbkine設計、開發(fā)、生產(chǎn)并銷售了一系列獨特的產(chǎn)品,包括生命科學領(lǐng)域研究相關(guān)的抗體、研究試劑以及細胞功能分析產(chǎn)品。Abbkine的產(chǎn)品為科學研究中的挑戰(zhàn)提供卓有成效的解決方案,從而為全球的科學家節(jié)省時間并提高研究效率。作為Abbkine的中國區(qū)域總代理,艾美捷科技有限公司憑借豐富的專業(yè)經(jīng)驗,將一如既往的為中國區(qū)域客戶提供Abbkine優(yōu)質(zhì)的產(chǎn)品和技術(shù)服務。
【英文名稱】:DHODH Polyclonal Antibody
【中文名稱】:二氫乳清酸脫氫酶 多克隆抗體
【產(chǎn)品類型】:一抗
【供應商】:Abbkine
【產(chǎn)品貨號】:ABP54006
【背景資料】:Dihydroorotate dehydrogenase encoded by DHODH catalyzes the fourth enzymatic step, the ubiquinone-mediated oxidation of dihydroorotate to orotate, in de novo pyrimidine biosynthesis. This protein is a mitochondrial protein located on the outer surface of the inner mitochondrial membrane. 蛋白別名為:DHODH; Dihydroorotate dehydrogenase; quinone); mitochondrial; DHOdehase; Dihydroorotate oxidase;基因ID為:1723;蛋白質(zhì)ID:Q02127
【產(chǎn)品規(guī)格】:30u/100ul/200ul
【應用類型】:WB,IHC-p,ELISA補充:最優(yōu)的抗體稀釋比例需要基于客戶實驗進行優(yōu)化.建議的起始稀釋比例如下: WB: 1:500-1:2000, IHC-p: 1:100-1:300, ELISA: 1:40000. Not yet tested in other applications.
【免疫原】:合成多肽:the Internal region of human DHODH. at AA rangle: 180-260
【來源宿主】:兔
【反應性】:人
【保存建議】:廠家推薦藍冰運輸.在發(fā)貨后能在-20℃保存1年.為最大限度的避免損失,請在打開管蓋之前融化抗體并離心.我們建議使用前分裝以避免反復凍融.本產(chǎn)品為液體形式,溶解于PBS緩沖液中,含50%甘油,0.5%BSA和0.02%疊氮化鈉.
【產(chǎn)品說明】:點擊:二氫乳清酸脫氫酶 多克隆抗體查看更詳細產(chǎn)品說明,更多應用、儲存、價格、貨期等信息請致電400-6800-857垂詢Abbkine中國區(qū)域授權(quán)總代理艾美捷科技有限公司。更多特色試劑盒、特色抗體以及特色試劑等產(chǎn)品評論,請點擊查看每日生物評論網(wǎng)站。
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兔子可溶性血管內(nèi)皮細胞蛋白C受體(sEPCR)ELISA試劑盒
使用目的: 為定量(性)測定活化的目標濃度的血清,血漿,組織勻漿,細胞培養(yǎng)上清及其他生物液體。研究使用不是用于診斷或治療程序。 如果你有任何問題,請聯(lián)系上海義森生物科技有限公司。
兔子可溶性血管內(nèi)皮細胞蛋白C受體(sEPCR)ELISA試劑盒
注意事項1. 嚴格按照規(guī)定的時間和溫度進行溫育以保證準確結(jié)果。所有試劑都必須在使用前達到室溫20-25℃。使用后立即冷藏保存試劑。2. 洗板不正確可以導致不準確的結(jié)果。在加入底物前確保盡量吸干孔內(nèi)液體。溫育過程中不要讓微孔干燥掉。3. 消除板底殘留的液體和手指印,否則影響OD值。4. 底物顯色液應呈無色或很淺的顏色,已經(jīng)變藍的底物液不能使用。5. 避免試劑和標本的交叉污染以免造成錯誤結(jié)果。6. 在儲存和溫育時避免強光直接照射。7. 平衡至室溫后再打開密封袋以防水滴凝聚在冷板條上。8. 任何反應試劑不能接觸漂白溶劑或漂白溶劑所散發(fā)的強烈氣體。任何漂白成分都會破壞試劑盒中反應試劑的生物活性。9. 不能使用過期產(chǎn)品。10. 如果可能傳播疾病,所有的樣品都應管理好,按照規(guī)定的程序處理樣品和檢測裝置。 試劑準備試劑盒從冷藏環(huán)境中取出應在室溫平衡后方可使用。1. 標準品復溶:試劑盒提供6管標準品,每管已標定濃度,并且凍干。實驗前在每個標準品管中加入0.5mL樣本稀釋液,蓋好后靜置10分鐘以上,然后反復顛倒/搓動助其溶解,使其恢復為每個標準品管身標注的濃度。2. 20×洗滌緩沖液的稀釋:蒸餾水按1:20稀釋,即1份20×洗滌緩沖液加19份蒸餾水。 兔子可溶性血管內(nèi)皮細胞蛋白C受體(sEPCR)ELISA試劑盒
操作步驟1. 從室溫平衡20min后的鋁箔袋中取出所需板條,剩余板條用自封袋密封放回4℃。2. 設置標準品孔和樣本孔,標準品孔各加不同濃度的標準品50μL;3. 樣本孔中加入待測樣本50μL;空白孔不加。4. 除空白孔外,標準品孔和樣本孔中每孔加入辣根過氧化物酶(HRP)標記的檢測抗體100μL,用封板膜封住反應孔,37℃水浴鍋或恒溫箱溫育60min。5. 棄去液體,吸水紙上拍干,每孔加滿洗滌液(350μL),靜置1min,甩去洗滌液,吸水紙上拍干,如此重復洗板4-5次(也可用洗板機洗板)。6. 每孔加入底物A、B各50μL,37℃避光孵育15min。7. 每孔加入終止液50μL,15min內(nèi),在450nm波長處測定各孔的OD值。 實驗結(jié)果計算以所測標準品的OD值為橫坐標,標準品的濃度值為縱坐標,在坐標紙上或用相關(guān)軟件繪制標準曲線,并得到直線回歸方程,將樣品的OD值代入方程,計算出樣品的濃度。
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